Anti-Human IgG (Fc specific), F(ab′)2 fragment, highly cross adsorbed antibody produced in goat
SIGMA/SAB3701274 - affinity isolated antibody, buffered aqueous solution
Product Type: Product-on-demand
Immunoblotting Anti-Human IgG (Fc specific), F(ab′)2 fragment: Cat. No. SAB3701274: Western Blot analysis of Goat Anti-F(ab′)2 Human IgG F(c) antibody pre-adsorbed. F(ab′)2 Human IgG F(c) antibody pre-adsorbed at 1:1,000 o/n at 4 °C and DyLight 800 Donkey Anti-Goat at 1:20,000 for 30 minutes at room temperature with Blocking Buffer for 30 minutes at room temperature. Predicted/Observed size: 28 kDa loaded at 100 ng per lane. Lane 1: Human IgG F(c).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect ELISA: suitable | |
| western blot: suitable |
| Biochem/physiol Actions: | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Anti-Pepsin, Anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse, Mouse and Rat Serum Proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antibody format: IgG F(ab′)2 |
| Immunogen: | Human IgG F(c) fragment |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |