Anti-Human IgG (Fc specific), highly cross adsorbed-Biotin antibody produced in goat
SIGMA/SAB3701279 - affinity isolated antibody, lyophilized powder
Synonym: Goat Anti-Human IgG
Product Type: Product-on-demand
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| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | biotin conjugate |
| form | lyophilized powder |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable |
| Application: | Anti-Human IgG (Fc specific)-Biotin antibody produced in goat (highly cross adsorbed) has been used in immunohistochemistry and for binding detection in ligand binding assay. |
| Biochem/physiol Actions: | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Biotin, Anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab’)2or Mouse serum proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antibody format: IgG |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family. It is widely expressed and consists of a gamma heavy chain in the constant (C) region. IgG is a monomer with a molecular weight of about 150 kDa. The structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc). It interacts with cells and effector molecules. The Fc fragment contains the CH2 and CH3 domains of the antibody molecule. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. |
| Immunogen: | Human IgG F(c) fragment |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preparation Note: | Reconstitute with 1.0 mL deionized water (or equivalent). |
| Symbol |   GHS06,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H311 - H411 |
| Precautionary statements | P280 - P301 + P312 + P330 - P302 + P352 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Supplemental Hazard Statements | EUH032 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |