Monoclonal Anti-Hsc70 (Hsp73) antibody produced in mouse
SIGMA/SAB3701436 - clone N27F3-4, purified immunoglobulin
Synonym: Anti-HSC54 antibody; Anti-HSC71 antibody; Anti-Heat shock protein 8 antibody; Anti-Heat-shock70-KD protein 10; Anti-MHV-A59
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-HSC70 (HSP73): Cat. No. SAB3701436: Western Blot analysis of Protein G purified Anti-Hsc70 (Hsp73) antibody shows detection of Hsc70 (Hsp73) in whole cell lysates from heat shocked HeLa cells. The band marked by the double arrowheads corresponds to Hsc70 (Hsp73) at an approximate molecular weight of 72/73 kDa. The primary antibody was used at a 1:1,000 dilution.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | N27F3-4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | canine, bovine, salmon, beluga, rat, Caenorhabditis elegans, human, carp, Drosophila, mouse, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P0DMV8  |
| Biochem/physiol Actions: | This Protein G purified monoclonal antibody reacts with human Hsc70 (Hsp73) protein. A BLAST analysis was used to suggest cross-reactivity with Hsc70 from human, bovine, mouse, rat, C.elegans, beluga, dog, chicken, Drosophila, carp, salmon, trout, guinea pig, hamster, monkey, pig, cucumber, pea, rabbit, sheep and Xenopus sources based on 100% homology with the immunizing sequence. This antibody recognizes both the inducible hsp and the constitutively expressed hsc as 72 kDa and 73 kDa proteins, respectively. Cross-reactivity with Hsp70 from other sources has not been determined. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to a region of human Hsc70 (Hsp73) protein. |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.1% (w/v) Sodium Azide |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |