Monoclonal Anti-HSP27 antibody produced in mouse
SIGMA/SAB3701437 - clone G3.1, purified immunoglobulin
Synonym: Anti-Heat shock 27 kDa protein antibody; Anti-Heat shock cognate protein 71-kDa antibody; Anti-HspB1 antibody; Anti-SRP27 antibody; Anti-
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-HSP27: Cat. No. SAB3701437: Western Blot analysis of Mab Anti-Human HSP27 antibody (clone G3.1) is shown to detect Human HSP27. Detection occurs after 10 μg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab Anti-Human HSP27 at room temperature for 30 min followed by detection using IRDye800 labeled Goat-Anti-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to Human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous Human HSP27.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | G3.1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| western blot: suitable | |
| UniProt accession no. | P04792  |
| Biochem/physiol Actions: | This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Prokaryotic recombinant protein corresponding to the full length human hsp27 protein. |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |