Monoclonal Anti-IDO1 antibody produced in mouse
SIGMA/SAB3701446 - clone 10.1, purified immunoglobulin
Synonym: Anti-3-dioxygenase 1; Anti-Ido1; Anti-Indo; Anti-Indoleamine 2; Anti-MIP-3?
Product Type: Product-on-demand
Immunoblotting Monoclonal Anti-IDO1: Cat. No. SAB3701446: Western Blot analysis of Mouse Anti-IDO1 antibody. Load: 35 μg per lane. Primary antibody: IDO 1 Antibody at 1:1,000 for overnight at 4 °C. Secondary antibody: IRDye800 Mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 °C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none. Lanes 1. Untreated HeLa cells 2. IFN-r treated HeLa cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 10.1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3 |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: suitable |
| immunohistochemistry: suitable | |
| immunoprecipitation (IP): suitable | |
| western blot: suitable | |
| UniProt accession no. | P14902  |
| Biochem/physiol Actions: | Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Fragment of recombinant human and mouse IDO1 protein followed by hybridoma development. |
| Physical form: | Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |