Anti-KRAS antibody, Mouse monoclonal
SIGMA/SAB4100599 - clone KRAS-A582, purified from hybridoma cell culture
Synonym: Anti-C-K-RAS; Anti-K-RAS2A; Anti-K-RAS2B; Anti-K-RAS4A; Anti-K-RAS4B
Product Type: Chemical
Immunofluorescence MCF7 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with Mouse Anti-KRAS Clone: KRAS-A582 (Cat. No. SAB4100599) diluted to 1:20. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Lysate of HEK-293T cells expressing FLAG-tagged human KRAS was separated on SDS-PAGE, probed with Mouse Anti-KRAS Clone: KRAS-A582 (Cat. No. SAB4100599). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250 2. Antibody dilution 1:500 3. Negative control 4. Positive control Anti-FLAG
Immunoblotting MCF7 cell extract was separated on SDS-PAGE and probed with Mouse Anti-KRAS Clone: KRAS-A582 (Cat. No. SAB4100599). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:50 2. Negative control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | KRAS-A582, monoclonal |
| conjugate | unconjugated |
| form | aqueous solution |
| isotype | IgG1 |
| mol wt | antigen predicted mol wt 27.9 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable using 5-10ug/ml using HEK-293 cells expressing KRAS |
| UniProt accession no. | P01116  |
| Biochem/physiol Actions: | Mutation in the KRAS gene causes non small-cell lung cancer and promotes tumor initiation, progression and metastasis. Altered KRAS gene also offers resistance to chemotherapy. It is involved in the signal transduction between epidermal growth factor receptor (EGFR) to the nucleus. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The kirsten rat sarcoma-2 virus oncogene (KRAS) gene is mapped to human chromosome 12p12.1 and belongs to the family of Ras genes. The protein encoded is a small guanosine nucleotide-bound GTPases belonging. |
| Immunogen: | KRAS purified recombinant protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |