Anti-POU1F1/PIT1 antibody produced in rabbit
SIGMA/SAB4200002 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GHF-1; Anti-
Product Type: Chemical
Immunoprecipitation Rabbit Anti-POU1F1/PIT1 (Cat. No. SAB4200002) was used to immunoprecipitate POU1F1/PIT1 from GH3 cell lysate. Detection antibody: 2 μg/mL Rabbit Anti-POU1F1/PIT1 (Cat. No. SAB4200002). Lanes 1. IP antibody: 5 μg 2. Negative control: without cell lysate 3. Negative control: without IP antibody 4. Immunoblotting positive control: total GH3 cell lysate
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~32 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using GH3 cell lysates |
| western blot: 1-2 μg/mL using GH3 cell lysates | |
| UniProt accession no. | P28069   |
| Application: | Anti-POU1F1/PIT1 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-POU1F1/PIT1 recognizes human POU1F1/PIT1. |
| Biochem/physiol Actions: | Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is responsible for pituitary development and hormone expression in mammals. It regulates mammalian development. POU1F1/PIT1 is required for the differentiation and proliferation of the anterior pituitary somatotrophs, lactotrophs and thyrotrophs cell lineages. Mutation in Pit-1 gene is associated with the syndrome of combined pituitary hormone deficiency (CPHD), a disease characterized by the lack of prolactin, growth hormone and thyroid stimulating hormone β. POU1F1/PIT1 regulates its target gene expression by binding to response elements on their promoter regions and recruitment of co-regulatory proteins, that alter histone acetylation and modify chromatin structures. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is a pituitary-specific transcription factor and is a member of the POU family of transcription factors. |
| General description: | POU1F1 is encoded by the gene mapped to human chromosome 3p11.2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |