Anti-DVL3 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200008 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Dishevelled-3
Product Type: Chemical
Immunoprecipitation Anti-DVL3 (N-terminal) (Cat. No. SAB4200008) was used to immunoprecipitate DVL3 from HEK-293T cells overexpressing human DVL3. Detection antibody: Anti-DVL3 (C-terminal region) (Cat. No. SAB4200007). Lanes 1. IP antibody: 5 μg 2. Negative control: without IP antibody 3. Negative control: without cell lysate
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-DVL3 (N-terminal) antibody produced in Rabbit (Cat. No. SAB4200008) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing DVL3 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 0.25 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human DVL3 (lanes 1,3) and of HEK-293T non transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-DVL3 (N-terminal) (Cat. No. SAB4200008) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 0.25 μg/mL 2. Antibody: 0.25 μg/mL 3. Antibody: 0.25 μg/mL + DVL3 immunizing peptide (human, 83-99)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~95 kDa |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using HEK-293T cell lysate overexpressing human DVL3 |
| western blot: 0.25-0.5 μg/mL using HEK-293T cell lysates overexpressing human DVL3 |
| Application: | Anti-DVL3 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-DVL3 (N-terminal) specifically recognizes human DVL3. |
| Biochem/physiol Actions: | Dishevelled 3 (DVL3) participates in the wingless-related integration site (Wnt) signaling pathway. It plays a key role in hippocampal neurogenesis regulation. Knockdown of DVL3 has been shown to attenuate the activation of the wnt signaling pathway by Wnt3A. DVL3 functions in redundant pathways with DVL1 and DVL2, and is necessary in normal cardiac, cochlea, and neural tube development in mice. DVL3, like other members of the DVL family, functions upstream of glycogen synthase kinase-3 (GSK3) to modulate the canonical wnt pathway. A frame shift mutation in DVL3 gene is implicated in Robinow syndrome. Allelic variation in the DVL3 gene may be associated with major depressive disorder. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dishevelled 3 (DVL3) is a phosphoprotein rich in serine and threonine. It is widely expressed in fetal tissues and specifically expressed in various adult tissues. DVL3 gene is mapped to human chromosome 3q27.1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |