Anti-MUC1 antibody , Mouse monoclonal
SIGMA/SAB4200017 - clone 1D1, purified from hybridoma cell culture
Synonym: Anti-CD227; Anti-EMA; Anti-H23AG; Anti-KL-6; Anti-MAM6; Anti-Mucin1; Anti-PEM; Anti-PEMT; Anti-PUM
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-MUC1 Clone: 1D1 (Cat. No. SAB4200017). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. T47D; 2. HeLa
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D1, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 160-500 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| radioimmunoassay: suitable | |
| western blot: 0.5-1.0 μg/mL using T47D cell lysates | |
| UniProt accession no. | P15941  |
| Application: | Monoclonal Anti-MUC1 antibody produced in mouse has been used in: • immunoblotting • enzyme linked immunosorbent assay (ELISA) • radioimmunoassay • immunoprecipitation • immunocytochemistry |
| Biochem/physiol Actions: | Mucin 1 (Muc1) exhibits protective action by binding to pathogens and is also associated with cell signaling capacity. Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. Muc1 might serve as a candidate for single chain antibody mediated targeted therapy in MUC1 expressing cancers. They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. Muc1 is a type I transmembrane glycoprotein with a unique extracellular domain containing 20 amino acid variable number tandem repeat (VNTR) domain. In many epithelia, this protein is attached to the apical surface by a transmembrane domain. The degree of glycosylation varies with cell type. The MUC1 gene is mapped to human chromosome 1q22. |
| Immunogen: | semi purified MUC1 from pleural effusion of a breast cancer patient. |
| Legal Information: | Purchase of this product conveys a non-exclusive license to use the product for research purposes only. Purchase of this product does not convey a right to (i) incorporate or use the product in the manufacture of any commercial product, (ii) sell or otherwise transfer the product to any third party, or (iii) use the product to provide any commercial service. THE PRODUCT IS NOT FOR IN VIVO HUMANS USE. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |