Anti-TES antibody ,Mouse monoclonal
SIGMA/SAB4200020 - clone TES75, purified from hybridoma cell culture
Synonym: Anti-TESS; Anti-TESS-2; Anti-Testin; Anti-Testis derived transcript
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-TES Clone: TES75 (Cat. No. SAB4200020). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Mouse thymus; 2. Mouse testes; 3. Mouse spleen; 4. K562; 5. HeLa; 6. MCF-7; 7. 3T3; 8. COS-7
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TES75, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG3 |
| mol wt | antigen ~48 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, mouse, human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1.0 μg/mL using whole extract of mouse thymus |
| UniProt accession no. | Q9UGI8  |
| Application: | Anti-TES antibody, mouse monoclonal may be used in immunoblotting. |
| Biochem/physiol Actions: | Monoclonal Anti-TES recognizes human, monkey, bovine, and mouse TES |
| Biochem/physiol Actions: | Testin LIM domain protein (TES), a putative tumor-suppressor gene, is essential for motility and is key for the tumor cell anchorage and metastasis. TES mRNA expression is found in several cancer-derived cell lines, particularly hematopoietic, breast and ovarian cancer cell lines as well as in primary tumors. This was correlated with methylation of TES CpG island. Furthermore, TES gene knockout revealed its role as a tumor suppressor gene as well as in actin stress fibre organization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-TES (mouse IgG3 isotype) is derived from the hybridoma TES75 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Testin LIM domain protein (TES) comprises cysteine-rich zinc-binding motifs called LIM domains and is a component of focal adhesions. TES gene is mapped to a common fragile site on chromosome 7q31.2 is designated as FRA7G. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |