Anti-VPS4B antibody produced in rabbit
SIGMA/SAB4200023 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-SKD1; Anti-SKD1-homolog; Anti-SKD1A; Anti-SKD2; Anti-VPS4; Anti-VPS4-1; Anti-Vacuolar protein sorting 4 homolog A; Anti-Vacuolar protein sorting factor 4A
Product Type: Chemical
Immunoprecipitation Rabbit Anti-VPS4B (Cat. No. SAB4200023), was used to immunoprecipitate VPS4B from 3T3 whole cell extract. Detection antibody: Rabbit Anti-VPS4 (Cat. No.SAB4200025) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate. Lanes 1. Positive control: 3T3 whole cell extract 2. 20 μg antibody 3. 5 μg antibody 4. Negative control: 5 μg nonrelevant antibody
Immunoblotting Whole extract of G361 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4B (Cat. No. SAB4200023). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + VPS4B immunizing peptide (human, 81-93) 3. 1 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using lysate of mouse 3T3 cells |
| western blot: 2-4 μg/mL using whole extracts of G361 cells | |
| UniProt accession no. | O75351  |
| Application: | Anti-VPS4B antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Vacuolar protein sorting 4 (Vps4) mediates endosomal membrane protein sorting, retroviral budding, and cytokinesis by recognizing membrane-associated endosomal sorting complexes required for transport (ESCRT-III) assemblies and catalysing their disassembly. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vacuolar protein sorting 4 homolog B (Vps4B) is a member of the AAA protein family (ATPases associated with diverse cellular activities) and is a homolog of yeast Vps4 protein. In humans, two of yeast protein paralogs have been identified as Vps4A and Vps4B. Both share high degree of amino acid sequence. The gene for Vps4B resides on human chromosome 18. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |