Anti-Notch 1 antibody, Rat monoclonal
SIGMA/SAB4200024 - clone ICN 5B5, purified from hybridoma cell culture
Synonym: Anti-Notch homolog 1, translocation-
Product Type: Chemical
Western Blotting Whole extract of Human Jurkat cells were separated on SDS-PAGE and probed with Monoclonal Anti-Notch 1 antibody produced in Rat, Clone: ICN 5B5 (Cat. No. SAB4200024). The antibody was developed using 1:10,000 Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9542). Lanes 1. 8 μg/mL antibody 2. 4 μg/mL antibody 3. 2 μg/mL antibody 4. 1 μg/mL antibody 5. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | ICN 5B5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2b |
| mol wt | antigen ~120 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using whole extract of human Jurkat cells and developing with a sensitive film |
| UniProt accession no. | P46531   |
| Application: | Monoclonal Anti-Notch 1 antibody produced in rat has been used in immunoblotting. |
| Biochem/physiol Actions: | In chronic obstructive pulmonary disease, notch receptor 1 (Notch1) helps to modulate endothelial apoptosis via the extracellular signal-regulated kinase (ERK) pathway. Overexpression of Notch1 helps to guard endothelial cell from apoptosis. Knockdown of Notch1 results in the enhancement of apoptotic cells. Notch1 serves as a tumor suppressor gene in mammalian epidermis and is a determinant of keratinocyte differentiation. Notch signaling is believed to participate in several human neoplasms including T-cell acute lymphoblastic leukemia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Notch 1 (rat IgG2b isotype) is derived from the hybridoma ICN 5B5 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a recombinant fusion protein corresponding to a fragment of human Notch 1 fusion protein. Notch receptor 1 (Notch1) gene is located on human chromosome 9q34. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |