Anti-VPS4 antibody produced in rabbit
SIGMA/SAB4200025 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Vacuolar protein sorting 4
Product Type: Chemical
Immunoprecipitation Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4 (Cat. No. SAB4200025) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate. Lanes 1. Positive control: HeLa whole cell extract 2. 10 μg antibody 3. 2 μg antibody
Immunoprecipitation Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4B (Cat. No. SAB4200023) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate. Lanes 1. 10 μg antibody 2. 2 μg antibody 3. Negative control: 10 μg nonrelevant antibody
Immunoprecipitation Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4A (Cat. No. SAB4200022) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate. Lanes 1. 10 μg antibody 2. 2 μg antibody 3. Negative control: 10 μg nonrelevant antibody
Immunoblotting Whole extract of NRK cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) 3. 1 μg/mL antibody
Immunoblotting Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) 3. 1 μg/mL antibody
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) 3. 1 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-4 μg using lysates of human HeLa cells |
| western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or rat NRK cells | |
| UniProt accession no. | Q9UN37   |
| Application: | Anti-VPS4 antibody has been used in • immunoprecipitation • immunofluorescence Confocal Microscopy • immunohistochemistry • western blotting |
| Biochem/physiol Actions: | Anti-VPS4 recognizes human, mouse, and rat VPS4A and VPS4B. |
| Biochem/physiol Actions: | Vacuolar protein sorting-associated protein 4 (VPS4) mediates endosomal membrane protein sorting, retroviral budding and cytokinesis by recognizing membrane-associated ESCRT-III assemblies and catalyzing their disassembly. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Vacuolar protein sorting-associated protein 4 (Vps4) is a member of the AAA protein family (ATPases associated with diverse cellular activities). Vps4 exists as two orthologs in humans (VPS4A and VPS4B). The gene encoding Vps4A is mapped to chromosome 16q22, while the gene for Vps4B resides on chromosome 18q21-q22. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |