Anti-ITCH antibody produced in rabbit
SIGMA/SAB4200036 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AIP4; Anti-
Product Type: Chemical
Immunoblotting Lysate of various cell lines (lanes 1-5), were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-ITCH (Cat. No. SAB4200036) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. HeLa 2. COS7 3. HEK-293T 4. Jurkat 5. Jurkat. Antibody + ITCH immunizing peptide (human, 161-179)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~112 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3.0 μg/mL using Jurkat and COS7 cell extracts |
| UniProt accession no. | Q96J02  |
| Application: | Anti-ITCH antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-ITCH specifically recognizes human and monkey ITCH. |
| Biochem/physiol Actions: | ITCH can act as a transcriptional corepressor of nuclear factor erythroid 2 (NFE2)/p45. This protein may participate in mediating the immune responses by modifying Notch-mediated signaling. |
| Biochem/physiol Actions: | Itchy E3 ubiquitin protein ligase (ITCH) in association with Ebola virus VP40 (eVP40), aids in virus-like particles (VLP) and virus budding. Overexpression of the gene is associated with the development of thyroid tumors, mainly anaplastic thyroid carcinoma (ATC). ITCH inhibits hippo tumor suppressor pathway and induce metastasis in breast cancer patients. The encoded protein is implicated in the positive regulation of transforming growth factor-β (TGF-β) signaling and of following epithelial-mesenchymal transition (EMT)-related gene expression. Down-regulated expression of the gene leads to syndromic multisystem autoimmune disease and severe morphologic and developmental abnormalities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Itchy E3 ubiquitin protein ligase (ITCH), also known as atrophin 1-interacting protein 4, is encoded by the gene mapped to human 20q11.22. The encoded protein is a member of the Nedd4-like family of E3 ubiquitin ligases. ITCH is characterized by an N- terminal protein kinase C-related C2 domain, four WW domains and a HECT (homologous to the E6-associated protein carboxyl-terminus) ubiquitin protein ligase domain at the C-terminus. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |