Anti-Talin antibody,Mouse monoclonal
SIGMA/SAB4200041 - clone TA205, purified from hybridoma cell culture
Synonym: Anti-TLN
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with 4 μg/mL Mouse anti Talin Clone:TA-205 (Cat. No. SAB4200041). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Western Blotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-Talin antibody produced in Mouse, Clone: TA205 (Cat. No. SAB4200041). The antibody was developed using 1:3,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A2304). Lanes 1. HeLa; 2. 293-T; 3. Jurkat; 4. COS-7; 5. NRK; 6. 3T3; 7. CHO; 8. MDBK; 9. MDCK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | TA205, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~225 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken, bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: 0.5-1 μg/mL using HeLa cells | |
| western blot: 0.5-1.0 μg/mL using HeLa/Jurkat cell extracts |
| Application: | Monoclonal Anti-Talin antibody produced in mouse has been used in western blotting and immunostaining. |
| Application: | Monoclonal Anti-Talin antibody produced in mouse has been used in: • Immunoprecipitation • immunofluorescence • immunocytochemistry |
| Biochem/physiol Actions: | Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent) -resistance. |
| Biochem/physiol Actions: | Talin 1 assists in the attachment of adherent cells to the extracellular matrix and of lymphocytes to other cells. It plays an important role in the assembly of actin filaments and in spreading and migration of different cell types, such as fibroblasts and osteoclasts. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the hybridoma TA205 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with partially purified preparation of human platelet talin. Talin is present in areas of cell-substratum and cell-cell contacts. Two types of talin were found in human, talin 1 and talin 2. Talin 1 co-distributes with integrins in the cell surface membrane. The C-terminus comprises binding sites for proteins such as β-1-integrin, actin, and vinculin. The N-terminus of the protein consists of elements for localization to cell extracellular matrix junctions. Talin 2 has a different pattern of expression compared to talin 1, but talin1 associates with unique transmembrane receptors to form novel linkages between extracellular matrices and actin cytoskeleton. |
| General description: | Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation. |
| General description: | Talin is a cytoskeletal protein that is found concentratedin areas of cell-substratum and cell-cell contacts. Theencoded protein plays a significant role in the assemblyof actin filaments and in spreading and migration ofvarious cell types, includ |
| Immunogen: | partially purified preparation of human platelet talin |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |