Anti-SRGAP3 antibody produced in rabbit
SIGMA/SAB4200050 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Wave-associated rac GTPase-activating protein ; Anti-ARHGAP14; Anti-MEGAP; Anti-Mental disorder-associated gap protein; Anti-SLIT-ROBO GAP 3; Anti-SLIT-ROBO Rho GTPase-activating protein 3 (SRGAP3); Anti-WRP
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-SRGAP3 antibody produced in Rabbit (Cat. No. SAB4200050) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing SRGAP3 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Tissue and cell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-SRGAP3 (Cat. No. SAB4200050) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Mouse brain (S1 fraction) 2. HEK-293T overexpressing human SRGAP3A 3. HEK-293T overexpressing human SRGAP3B 4. HEK-293T non transfected 5. Mouse brain (S1 fraction) + SRGAP3 immunizing peptide (human, 897-915)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~124 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3 μg/mL using mouse brain extracts (S1 fraction) and HEK-293T cell lysates overexpressing SRGAP3 |
| UniProt accession no. | O95747  |
| Application: | Anti-SRGAP3 antibody produced in rabbit has been used in immunofluorescence and immunoblotting. |
| Biochem/physiol Actions: | SRGAP3 (Slit-ROBO Rho GTPase-activating protein 3) negatively regulates cell migration, by inhibiting the activity of Rac1 and Cdc42. SRGAP3/MEGAP has been associated with severe mental retardation in several patients. It plays pivotal roles in learning and memory. SRGAP3/WRP interacts directly to WAVE-1 through its Src homology domain and specifically inhibits Rac function in vivo.Induced expression of SRGAP3 inhibits Rac1 and Cdc42 activity and impedes cell migration by impairing actin and microtubule dynamics and formation of focal complexes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-SRGAP3 is produced in rabbit using as the immunogen a synthetic peptide corresponding to a fragment of human SRGAP3 (GeneID: 9901), conjugated to KLH. SRGAP3 (Slit-ROBO Rho GTPase-activating protein 3, also known as MEGAP, is a GTPase activating protein, that belongs to the Slit-ROBO GAPs (SRGAPs) family. It is predominantly expressed in the brain during embryonic development and in the adult brain, specifically in neurons of the hippocampus and cortex. SRGAP3 exists as two isoforms SRGAP3A and SRGAP3B. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |