Anti-DNA polymerase δ p50 antibody, Rat monoclonal
SIGMA/SAB4200054 - clone PDK 7B4, purified from hybridoma cell culture
Synonym: Anti-POLD2; Anti-Polymerase (DNA directed), delta 2, regulatory subunit 50kDa; Monoclonal Anti-DNA polymerase δ p50 antibody produced in rat
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Rat Anti-DNA polymerase p50 Clone: PDK 7B4 (Cat. No. SAB4200054). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. HeLa; 2. 293T; 3. Jurkat; 4. COS7; 5. NRK; 6. 3T3; 7. CHO
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | PDK 7B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.5-1.0 μg/mL using HeLa cell extracts | |
| UniProt accession no. | P49005  |
| Application: | Monoclonal Anti-DNA polymerase δ p50 antibody has been used in • immunoblotting • immunoprecipitation • immunocytochemistry • western blot analysis |
| Biochem/physiol Actions: | DNA polymerase δ 2 subunit (POLD2) gene codes for a protein that participates in DNA replication and repair. In COS-7 cells, regulatory region of the hPOLD2 gene has ability to facilitate the expression of chloramphenicol acetyltransferase reporter gene. Phosphatase and tensin homolog (PTEN) tumor suppressor gene decrease the expression of POLD2 gene associated with ovarian carcinogenesis. This protein might also function as a potential prognostic biomarker in ovarian carcinoma. POLD2 takes part in polymeraseδ (Pol δ) assembly by coordinating simultaneously with all other three subunits of the enzyme. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-DNA polymerase δ p50 (rat IgG2a isotype) is derived from the hybridoma PDK 7B4 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p50. DNA polymerase δ consists of the proliferating cell nuclear antigen (PCNA), the multi-subunit replication factor C, and the 4 subunit DNA polymerase complex: DNA Polymerase δ 1, 2, 3, 4 (POLD1, POLD2, POLD3 and POLD4). The core DNA polymerase δ exists as a heterodimeric enzyme with subunits of 125 and 50 kDa. |
| General description: | The DNA polymerase δ 2 subunit (POLD2) gene, with 11 exons spanning 10kb of genomic DNA, is mapped to human chromosome 7. The gene encoding 50kDa protein is characterized with the G+C-rich 5′-flanking region and it lacks a typical TATA box. The encoded protein is one of the components of human DNA Pol δ complex. |
| Immunogen: | human p50 |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |