Anti-CYLD antibody produced in rabbit
SIGMA/SAB4200060 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Cylindromatosis (turban tumor syndrome), deubiquitinating enzyme CYLD; Anti-Ubiquitin carboxyl-terminal hydrolase CYLD; Anti-Ubiquitin specific peptidase like 2; Anti-Ubiquitin thiolesterase CYLD
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human skin sections with 20 μg/mL Rabbit Anti-CYLD (Cat. No. SAB4200060) using biotin/ExtrAvidin®-Peroxidase.
Immunoprecipitation Rabbit Anti-CYLD (Cat. No. SAB4200060) was used to immunoprecipitate CYLD from human HEK-293T whole cell extract. Detection antibody: Rabbit Anti-CYLD (C-terminal) (Cat. No. SAB4200061). Lanes 1. 10 μg antibody 2. 20 μg antibody 3. Negative control: 10 μg non-relevant antibody
Immunoblotting Whole extract of mouse brain was separated on SDS-PAGE and probed with Rabbit Anti-CYLD (Cat. No. SAB4200060). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
Immunoblotting Whole extract of rat brain was separated on SDS-PAGE and probed with Rabbit Anti-CYLD (Cat. No. SAB4200060). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~110 kDa |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human skin sections and biotin / ExtrAvidin®-Peroxidase staining system |
| western blot: 1-2 μg/mL using whole extracts of rat and mouse brain | |
| UniProt accession no. | Q9NQC7   |
| Application: | Anti-CYLD antibody can be used in immunohistochemistry (10-20 μg/mL using heat-retrieved, formalin-fixed, paraffin-embedded, human skin sections and biotin / ExtrAvidin® -Peroxidase staining system). This antibody is also suitable for use in immunoblot (approx. 110 kDa) and western blot (1-2 μg/mL using whole extracts of rat and mouse brain). |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Cylindromatosis (CYLD) physically interacts with many different proteins and mediates signaling through the nuclear factor kappa-light-chain-enhance |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-CYLD is produced in rabbit using as the immunogen a synthetic peptide corresponding to a fragment of human CYLD conjugated to KLH. Cylindromatosis (CYLD) contains an ubiquitin C-terminal ubiquitin-specific protease (USP) catalytic domain, which is responsible for the removal of ubiquitin chains and three cytoskeleton-associated protein-glycine conserved (CAP-Gly) domains. |
| General description: | CYLD is a tumor suppressor that encodes a deubiquitination enzyme. This enzyme regulates cell growth and survival. Inhibition of CYLD can cause cancers of the skin appendages, namely, multiple trichoepithelioma and familial cylindromatosis . Anti-CYLD antibody is specific for CYLD in humans, rats and mice. The immunizing peptide specifically inhibits the detection of the CYLD band by immunoblotting. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |