Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200066 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Argonaute-1; Anti-Eukaryotic translation initiation factor 2C, subunit 1; Anti-GERP95; Anti-Golgi Endoplasmic Reticulum protein 95 kDa
Product Type: Chemical
Immunofluorescence Cytoplasmic P-bodies localization of eIF2C1/Ago1 in HEK-293T cells over-expressing FLAG-tagged eIF2C1/Ago1. Cells were fixed and co-stained with 5 μg/mL Rabbit Anti-eIF2C1/Ago1 (N-Terminal) (Cat. No. SAB4200066) (Red) and 1:1,000 Anti-FLAG (Cat. No. F3165) (Green) followed by Goat Anti-Rabbit, Cy3 conjugated and Goat Anti-Mouse IgG, Atto 488 conjugated (Cat. No. 62197) respectively. The cells were counterstained with DAPI (Blue) to stain nuclei (×40 magnification).
Immunoblotting Lysate of HEK-293T cells over-expressing human eIF2C1 /Ago1 was separated on SDS-PAGE and probed with 1 μg/mL Anti-eIF2C1/Ago1 (N-terminal) (Cat. No. SAB4200066). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 1 μg/mL + 20 μg/mL Anti-eIF2C1/Ago1 (N-terminal) immunizing peptide
Immunoprecipitation Rabbit Anti-eIF2C1/Ago1 (N-terminal) (Cat. No. SAB4200066) was used to immunoprecipitate human eIF2C1/Ago1 from lysate of HEK-293T cells over-expressing human eIF2C1/Ago1. Detection antibody: 4 μg/mL Anti-eIF2C1/Ago1 (Cat. No. SAB4200065). Lanes 1. IP antibody: 10 μg 2. Negative control: without cell lysate 3. Negative control: without IP antibody 4. Immunoblotting positive control: total HEK-293T cells over-expressing human eIF2C1/Ago1. Detection antibody: 2 μg/mL Anti-eIF2C1/Ago1 (N-terminal) (Cat. No. SAB4200066)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~100 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over expressing hEIF2C1/Ago1 |
| indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing hEIF2C1/Ago1 | |
| western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing hEIF2C1/Ago1 |
| Application: | Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has also been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Application: | Anti-EIF2C1/Ago1 (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) and RNA-native ChIP (NChIP) experiments. |
| Biochem/physiol Actions: | EIF2C1 is implicated in both transcriptional and post-transcriptional gene silencing. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene, WT1. |
| Biochem/physiol Actions: | Eukaryotic initiation factor (EIF2C1)/ argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In humans, the argonaute (Ago) subfamily consists of four isoforms hsAgo1−4. Ago proteins (~100 kDa molecular weight) contain a piwi-argonaute-zwille (PAZ) and PIWI domains. These proteins are evolutionarily conserved between species. EIF2C1/Ago1 is predominantly expressed in embryonic kidney and lung. |
| General description: | The eukaryotic initiation factor (EIF2C1), also known as argonaute 1, RNA-induced silencing complex (RISC) catalytic component, is encoded by the gene with 19 exons and 18 introns spanning ~ 50 kb on genomic DNA, mapped to human chromosome 1p34.3. The gene is widely expressed at low and medium levels. The encoded protein is a member of argonaute family of proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |