Anti-VAC14 antibody produced in rabbit
SIGMA/SAB4200074 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ArPIKfyve; Anti-TAX1-binding protein 2; Anti-TAX1BP2; Anti-TAX1BP2 TAX-reactive protein x; Anti-TRX
Product Type: Chemical
Immunofluorescence Cytoplasmic and nuclear localization of VAC14 in HeLa cells. Fixed cells were stained with 8 μg/mL Anti-VAC14 (Cat. No. SAB4200074) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoprecipitation Anti-VAC14 (Cat. No. SAB4200074), was used to immunoprecipitate human VAC14 from A431 cell lysate. Detection antibody: Anti-VAC14 (C-terminal) (Cat. No. SAB4200075). Lanes 1. IP antibody: 10 μg, (50 μg cell lysate) 2. IP antibody: 10 μg, (30 μg cell lysate) 3. Negative control: without IP antibody 4. Negative control: without cell lysate 5. IB control: A431 cell lysate. Detection antibody: Anti-VAC14 (Cat. No. SAB4200074)
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-VAC14 (Cat. No. SAB4200074), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HeLa 2. HEK293-T 3. HepG2 4. A431 5. HeLa. Antibody + VAC14 immunizing peptide (human, 341-359)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~82 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-15 μg using A431 cell lysates |
| indirect immunofluorescence: 8-16 μg/mL using HeLa cells | |
| western blot: 1.5-3.0 μg/mL using HeLa cell lysates | |
| UniProt accession no. | Q08AM6   |
| Application: | Anti-VAC14 antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | VAC14 is a regulator of phosphoinositide kinase, FYVE-type zinc finger containing (PIP5K3/PIKfyve), a dual specificity kinase that phosphorylates phosphatidylinositol 3-phosphate (PtdIns3P). This generates housekeeping phospholipid PtdIns(3,5)P2, that controls multivesicular body morphology, retrograde traffic to the trans-Golgi network and is critical for neuronal survival. VAC14/ArPIKfyve upregulates PIKfyve phosphoinositide-5-kinase activity. In 3T3-L1 adipocytes, VAC14 and PIP5K3 interaction has been shown to play a critical role in insulin-regulated glucose transporter (GLUT4) translocation from intracellular storage compartment to the cell surface. Knockout of the VAC14 gene in mouse results in massive neurodegeneration and affects neurons in the midbrain and of the peripheral sensory system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | VAC14 (VAC14 component of PIKFYVE complex) gene (also known as ArPIKfyve, TAX1BP2, TRX) is mapped within human chromosome 16q22.1-q22.2 and encodes a scaffold protein. This protein is a component of phosphoinositide kinase, FYVE-type zinc finger containing (PIKfyve) protein kinase complex. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |