Anti-Vinculin antibody, Mouse monoclonal
SIGMA/SAB4200080 - clone V284, purified from hybridoma cell culture
Synonym: Anti-CMD1W; Anti-MVCL; Anti-VCL
Product Type: Chemical
Western Blotting JOURNAL CITATION: MELK is not necessary for the proliferation of basal-like breast cancer cells. By: Huang, H. T., Seo, H. S., et al. in Elife, 2017. PubMed ID: 28926338 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/26693), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: MELK is not necessary for the proliferation of basal-like breast cancer cells. By: Huang, H. T., Seo, H. S., et al. in Elife, 2017. PubMed ID: 28926338 Image collected and cropped by CiteAb from the following publication, (https://elifesciences.org/articles/26693), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence HeLa cells were fixed and permeabilized with cold Methanol followed by cold Methanol:Acetone (1:1). Fixed cells were stained with 4 μg/mL Mouse Anti-Vinculin Clone: V284 (Cat. No. SAB4200080). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Immunoblotting Lysates were separated on SDS-PAGE and probed with 0.015 μg/mL Mouse Anti-Vinculin Clone: V284 (Cat. No. SAB4200080). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. A431; 3. 3T3; 4. Neuro 2a; 5. PC12; 6. CFB; 7. MDCK; 8. MDBK; 9. COS7
| antibody form | purified from hybridoma cell culture |
| purified immunoglobulin | |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | V284, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~117 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rabbit, chicken, human, rat, monkey, mouse, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 4-8 μg/mL using HeLa cells fixed with methanol:acetone |
| immunoprecipitation (IP): suitable | |
| western blot: 0.005-0.01 μg/mL using HeLa total cell extracts | |
| UniProt accession no. | P18206  |
| Application: | Anti-Vinculin antibody, Mouse monoclonal has been used: • to probe blots in immunoblotting • immunochemical staining • immunoprecipitation • immunocytochemistry |
| Application: | Monoclonal Anti-Vinculin antibody produced in mouse has been used in western blotting. |
| Biochem/physiol Actions: | Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading. In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hybridoma V284 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with purified human platelet vinculin. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), neuromuscular junctions, myotendinous junctions (skeletal muscle) and the membrane-associated dense bodies of smooth muscle. |
| General description: | Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein. |
| Immunogen: | purified human platelet vinculin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |