Monoclonal Anti-BOP1 antibody produced in rat
SIGMA/SAB4200081 - ~1.0 mg/mL, clone BOP 6H11, purified immunoglobulin, buffered aqueous solution
Synonym: Anti-Block of proliferation 1
Product Type: Chemical
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Rat Anti-Bop1 Clone: Bop1 6H11 (Cat. No. SAB4200081). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. Negative control
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1 μg/mL Rat Anti-Bop1 Clone: Bop 6H11 (Cat. No. SAB4200081). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. MDBK; 2. NRK; 3. BHK; 4. CFB; 5. MDCK; 6. CHO; 7. PC12; 8. 3T3; 9. HeLa
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | BOP 6H11, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~100 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.5-1.0 μg/mL using HeLa cell extracts | |
| UniProt accession no. | Q14137   |
| Application: | Monoclonal Anti-BOP1 antibody produced in rat has been used in several immunochemical techniques including immunoblotting, immunoprecipitation, and immunocytochemistry. |
| Biochem/physiol Actions: | Ribosome biogenesis protein BOP1 is an essential component of A nucleolar complex, PeBoW that is involved in ribosome biogenesis and cell proliferation. Fluctuations in Bop1 protein level were found to affect the assembly and integrity of the whole PeBoW (Pes1, Bop1, and WDR12) complex. Overexpression of Bop1 mRNA is associated with the development of colorectal cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- BOP1 (rat IgG1 isotype) is derived from the hybridoma BOP 6H11 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to a fragment of human BOP1, coupled to ovalbumin. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |