Monoclonal Anti-AGO2 antibody produced in rat
SIGMA/SAB4200085 - ~1.5 mg/mL, clone 11A9, purified immunoglobulin
Synonym: AGO2 Antibody - Monoclonal Anti-AGO2 antibody produced in rat; Ago2 Antibody; Anti-Argonaute 2; Anti-EIF2C2 eukaryotic translation initiation factor 2C, 2; Anti-Q10
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1 μg/mL Rat Anti-AGO2 Clone: 11A9 (Cat. No. SAB4200085). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. HeLa; 2. MDBK; 3. COS7
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 11A9, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~85 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, rat, human, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 0.5-1.0 μg/mL using HeLa cell extracts | |
| UniProt accession no. | Q9UKV8  |
| Application: | Monoclonal Anti-AGO2 antibody produced in rat has been used in: • immunofluorescent localization • western blot analysis • immunoprecipitation. |
| Biochem/physiol Actions: | Argonaute 2 (Ago2) plays a vital role in short interfering RNA-mediated gene silencing. The encoded protein is regulated at both the transcriptional and posttranslational level in human breast cancer cell lines. Ago2 and enhanced micro-RNA activity is involved in the tumorigenic progression of breast cancer cell lines. Overexpression of the gene has been observed in hepatocellular carcinoma (HCC), head and neck squamous cell carcinoma patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-AGO2 (rat IgG2a isotype) is derived from the hybridoma 11A9 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a peptide corresponding to amino acids 1-15 of human AGO2. Monoclonal Anti-AGO2 recognizes human, monkey, bovine, rat and mouse AGO2. Argonaute 2 (Ago2) is encoded by the gene mapped to human chromosome 8q24.3. The encoded protein belongs to the Ago protein family. AGO2 is mainly localized on the diffuse cytoplasm and stress granules. |
| Other Notes: | Cell lysates should not be boiled before loading onto SDS-PAGE, but only warmed to 60°C for 15 min. In order to obtain the best results using various techniques and preparations, we recommend determining optimal working dilutions by titration. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |