Anti-DGCR8 (N-terminal) antibody produced in rabbit
SIGMA/SAB4200089 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-DGCRK8; Anti-DiGeorge syndrom critical region 8
Product Type: Chemical
Immunofluorescence Nuclear localization of DGCR8 in HEK-293T cells over-expressing human DGCR8. Cells were stained with 5 μg/mL Rabbit Anti-DGCR8 (N-Terminal) (Cat. No. SAB4200089) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei. (×40 magnification).
Immunoprecipitation Rabbit Anti-DGCR8 (N-Terminal) (Cat. No. SAB4200089) was used to immunoprecipitate human DGCR8 from lysate of HEK-293T cells over-expressing human DGCR8. Detection antibody: Anti-DGCR8 (C-terminal) (Cat. No. SAB4200088). Lanes 1. IP antibody: 5 μg 2. IP antibody: 2.5 μg 3. Negative control: IP antibody without cell lysate 4. Negative control: without IP antibody 5. Immunoblotting positive control: total HEK-293T cells over-expressing human DGCR8 blot with Anti-DGCR8 (N-terminal) (Cat. No. SAB4200089)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~100 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human DGCR8 |
| indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8 | |
| western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human DGCR8 | |
| UniProt accession no. | Q8WYQ5   |
| Application: | Anti-DGCR8 (N-terminal) antibody produced in rabbit has been used in western blotting. |
| Application: | Anti-DGCR8 (N-terminal) antibody produced in rabbit has been used in: • western blotting • immunoprecipitation • immunofluorescence |
| Application: | Anti-DGCR8 (N-terminal) antibody produced in rabbit is suitable for immunoprecipitation (2.5-5μg using lysates of HEK-293T cells over expressing human DGCR8), indirect immunofluorescence (2-5μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8) and western blot at a dilution of 1-2μg/mL (using lysates of HEK-293T cells over expressing human DGCR8). |
| Biochem/physiol Actions: | DGCR8 (DGCR8 microprocessor complex subunit) participates in the biogenesis of microRNA (miRNA, miR) as a major component of the microprocessor complex. DGCR8 provides guidance to the RNase III enzyme, Drosha during rRNA processing. It forms the microprocessor complex by binding to the RNase III enzyme Drosha, which further converts long primary miRNAs (pri-miRNAs) into short hairpins called precursor miRNAs (pre-miRNAs). The processed hairpins finally are transported to the cytoplasm for further processing by Dicer into mature miRNAs. DGCR8 is also required for global gene regulation and silencing of embryonic stem cell self-renewal. Deletion of DGCR8 chromosomal location has been reported in the DiGeorge and velocardiofacial syndrome. |
| Biochem/physiol Actions: | DGCR8, also known as DiGeorge syndrome critical region 8, DGCRK8 is the cofactor that interacts with drosha and forms a functional complex called the ‘‘Microprocessor” which is essential for microRNA (miRNA) maturation. DGCR8 contains an N-terminal region which is critical for nuclear localization and the C-terminal region stably interacts with the pri-miRNAs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DGCR8 (DGCR8 microprocessor complex subunit) is a double-stranded RNA-binding protein mapped to the chromosome 22q11.2. It is composed of a WW domain and two double-stranded RNA-binding domains (dsRBDs). |
| General description: | DGCR8 contains an N-terminal region which is critical for nuclear localization and C-terminus which can directly and stably interact with the pri-mRNAs. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |