Anti-PKM2 (isoform M1) antibody produced in rabbit
SIGMA/SAB4200094 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-CTHBP; Anti-OIP3 (OPA-interacting protein 3); Anti-PK3; Anti-PKM; Anti-Pyruvate Kinase, MUSCLE (isoform M1); Anti-TCB; Anti-THBP1 (thyroid hormone-binding protein, cytosolic)
Product Type: Chemical
Immunohistochemistry Cytoplasmic localization of PKM2 (isoform M1) in human colon. Formalin-fixed paraffin-embedded tissue was stained with 10 μg/mL Anti-PKM2 (isoform 1) (Cat. No. SAB4200094), followed by Goat Anti-Rabbit IgG, HRP conjugate (×40 magnification).
Immunoprecipitation Anti-PKM2 (isoform 1) (Cat. No. SAB4200094), was used to immunoprecipitate rat PKM2 (isoform 1) from rat brain extract (S2 fraction). Detection antibody: Anti-PKM2 (C-terminal) (Cat. No. SAB4200105), Lanes 1. IP antibody: 2 μg 2. Negative control: without cell lysate 3. Negative control: without IP antibody 4. IB control: rat brain extract (S2 fraction). Detection antibody: Anti-PKM2 (isoform 1) (Cat. No. SAB4200094)
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-PKM2 (isoform M1) antibody produced in Rabbit (Cat. No. SAB4200094) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing PKM2 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1 μg/mL.
Immunoblotting Cell and tissue extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-PKM2 (isoform 1) (Cat. No. SAB4200094), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. C2C12; 2. HEK-293T overexpressing human PKM2-M1; 3. HEK-293T non transfected; 4. Rat brain (S2 fraction); 5. mouse skeletal muscle; 6. rat skeletal muscle; 7. mouse brain (S2 fraction); 8. mouse brain (S2 fraction). Antibody + PKM2 (isoform 1) immunizing peptide (human, 398-416)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 5-10 μg/mL using HeLa cells |
| immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon | |
| immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction) | |
| western blot: 1-2 μg/mL using mouse brain extract (S2 fraction) |
| Application: | Anti-PKM2 (isoform M1) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunohistochemistry • immunocytochemistry |
| Biochem/physiol Actions: | Anti-PKM2 (isoform M1) antibody is specific for human, mouse and rat PKM2 (Isoform M1)/PKM1. In immunoblotting, detection of the PKM2 (Isoform M1) /PKM1 band is specifically inhibited by the immunizing peptide. |
| Biochem/physiol Actions: | Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. The M2 isoform has been linked to cancer metastasis. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |