Anti-Gapex-5 antibody produced in rabbit
SIGMA/SAB4200098 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-GAPVD1; Anti-
Product Type: Chemical
Immunoprecipitation Rabbit Anti-Gapex-5 (N-terminal) (Cat. No. SAB4200099) was used to immunoprecipitate Gapex-5 from HEK-293T whole cell extract. Detection antibody: Rabbit anti Gapex-5 (Cat. No. SAB4200098) (lanes 1-3). Rabbit Anti-Gapex-5 (Cat. No.SAB4200098) was used to immunoprecipitate Gapex-5 from HEK-293T whole cell extract. Detection antibody: Rabbit Anti-Gapex-5 (N-terminal) (Cat. No. SAB4200099) (lanes 4-6). Lanes 1, 4. 5 μg antibody 2, 5. 10 μg antibody 3, 6. Negative control: 5 μg nonrelevant antibody
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-Gapex-5 (Cat. No. SAB4200098). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~180 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of human HEK-293T cells |
| western blot: 1-2 μg/mL using whole extracts of human HeLa cells | |
| UniProt accession no. | Q14C86   |
| Application: | Anti-Gapex-5 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-Gapex-5 recognizes human Gapex-5. |
| Biochem/physiol Actions: | Rab5-activating protein 6 (RAP6) or Gapex-5 is an evolutionary conserved protein and its C-terminal Vps9 domain is responsible for the interaction with guanosine diphosphate GDP bound Rab5 and an N-terminal Ras GTPase-accelerating proteins (GAP) domain. Gapex-5 has guanine nucleotide exchange factor (GEF) activity specific for Rab5 and GAP activity specific for Ras. It is involved in multiple processes including receptor-mediated endocytosis, insulin receptor internalization and glucose transporter type 4 (Glut4) trafficking in adipocytes. RAP6 is also essential for epidermal growth factor receptor (EGFR) trafficking and degradation and phagosome maturation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GTPase-activating protein and VPS9 domain-containing protein 1 (GAPVD1) also called Rab5-activating protein 6 (RAP6) or Gapex-5 gene is mapped to the human chromosome 9q33.3. It has a carboxy-terminal Vps9-like domain and an amino-terminal Ras GTPase-accelerating proteins (GAP)-like domain. GAPVD1 is present in the plasma membrane and endosomes. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |