Anti-ATG13 antibody produced in rabbit
SIGMA/SAB4200100 - ~1.0 mg/mL, affinity isolated antibody
Synonym: ATG13 Antibody - Anti-ATG13 antibody produced in rabbit; Atg13 Antibody; Anti-
Product Type: Chemical
Immunoblotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-ATG13 (Cat. No. SAB4200100). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + ATG13 immunizing peptide (human, 462-475)
Immunoblotting Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-ATG13 (Cat. No. SAB4200100). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + ATG13 immunizing peptide (human, 462-475)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~65 kDa |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells. |
| Application: | Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy. |
| Biochem/physiol Actions: | Anti-Atg13 recognizes human, mouse, and rat Atg13. |
| Biochem/physiol Actions: | Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |