Anti-AS160 antibody produced in rabbit
SIGMA/SAB4200101 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-Akt substrate of 160 kDa; Anti-TBC1D4 (TBC1 domain family, member 4)
Product Type: Chemical
Immunofluorescence Cytoplasmic localization of AS160 in Hela cells. Fixed cells were stained with 8 μg/mL Anti-AS160 (Cat. No. SAB4200101) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-AS160 antibody produced in Rabbit (Cat. No. SAB4200101) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing AS160 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human AS160 (lanes 1,2,4) and HEK-293T non transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-AS160 (Cat. No. SAB4200101) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 0.75 μg/mL 3. Antibody: 1.5 μg/mL 4. Antibody: 1.5 μg/mL + AS160 immunizing peptide (human, 338-355)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~160 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 8-16 μg/mL using HEK-293T cells |
| western blot: 0.8-1.6 μg/mL using extracts of HEK-293T cells over expressing human AS160 | |
| UniProt accession no. | O60343  |
| Application: | Anti-AS160 antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | AS160 is known to play an inhibitory role in the transport of glucose transporter 4 (GLUT4) vesicles. It is associated with GLUT4 vesicles in basal states and dissociates in response to insulin. Reduced expression of AS160 in adipocytes has been shown to increase plasma membrane levels of GLUT4 in an insulin-dependent manner. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | AS160 (Akt substrate of 160 kDa, also known as TBC1 domain family member 4 (TBC1D4)) gene is mapped to human chromosome 13q22.2 and encodes a Rab guanosine triphosphatase (GTPase)-activating proteins (RabGAP) protein. AS160 is expressed in many differentiated tissues such as adipocytes and skeletal muscles. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |