Anti-ULK1 antibody produced in rabbit
SIGMA/SAB4200106 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-ATG1 autophagy related 1 homolog; Anti-UNC51; Anti-Unc51.1; Anti-unc-51-like kinase 1
Product Type: Chemical
Western Blotting JOURNAL CITATION: Gemcitabine induces poly (ADP-ribose) polymerase-1 (PARP-1) degradation through autophagy in pancreatic cancer. By: Wang, Y., Kuramitsu, Y., et al. in PLoS One, 2014. PubMed ID: 25271986 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0109076), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Recombinant Expression Western Blotting. Antibody recognition: Anti-ULK1 antibody produced in Rabbit (Cat. No. SAB4200106) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing ULK1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 2 μg/mL.
Immunoblotting Whole extract of HEK-293T cells overexpressing human ULK1 was separated on SDS-PAGE and probed with Rabbit Anti-ULK1 (Cat. No. SAB4200106). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1.1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + ULK1 immunizing peptide (human, 1-13)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~150 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human ULK1. |
| UniProt accession no. | O75385  |
| Application: | Anti-ULK1 has been used in immunoblotting. |
| Biochem/physiol Actions: | Anti-ULK1 recognizes human ULK1. |
| Biochem/physiol Actions: | Uncoordinated 51-like kinase 1 (ULK1) is involved in modulating Atg9 subcellular dynamics. Knockdown of ULK1, but not ULK2, inhibits the autophagic response. Overexpression of ULK1 also inhibits autophagy, suggesting that ULK1 may have multiple mechanisms for regulating autophagy. Knockdown of Ulk1 shRNA can prevent gastric cancer cell survival. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Uncoordinated 51-like kinase 1 (ULK1) is expressed in skeletal muscle, heart, pancreas, brain, placenta, liver, kidney and lung. This gene is located on human chromosome 2q24.3. ULK1 localizes to cytoplasmic structures. ULK1 and ULK2 is a homolog of autophagy related 1 (Atg1) gene. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |