Anti-EIF2C3/Ago3 antibody produced in rabbit
SIGMA/SAB4200112 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-Argonaute 3; Anti-Eukaryotic translation initiation factor 2C, subunit 3
Product Type: Chemical
Immunofluorescence Cytoplasmic P-bodies staining of EIF2C3/AGO3 in HEK-293T cells over expressing FLAG®-tagged EIF2C3/AGO3. Cells were fixed and co-stained with: A, 2.5 μg/mL Rabbit Anti-EIF2C3/AGO3 (Cat. No. SAB4200112) (Red) and B, 1:1,000 Anti-FLAG® (Cat. No. F3165) (Green) followed by Goat Anti-Rabbit, Cy3 conjugated and Goat Anti-Mouse IgG, Atto 488 conjugated (Cat. No. 62197) respectively. C, Overlay of A and B (×40 magnification).
Immunoblotting Lysate of HEK-293T cells over-expressing human EIF2C3/AGO3 was separated on SDS-PAGE and probed with Rabbit Anti-EIF2C3/AGO3 (Cat. No. SAB4200112). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 0.5 μg/mL 3. Antibody 0.25 μg/mL 4. Antibody 0.5 μg/mL + 1 μg/mL EIF2C3/AGO3 immunizing peptide
Immunoprecipitation Rabbit Anti-EIF2C3/AGO3 (Cat. No. SAB4200112) was used to immunoprecipitate human EIF2C3/AGO3 from Lysate of HEK-293T cells over-expressing EIF2C3/AGO3. Detection antibody: 1 μg/mL Monoclonal Anti-FLAG® (Cat. No. F3165) Lanes 1. IP antibody: 5 μg 2. IP antibody: 2.5 μg 3. Negative control: without IP antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~97 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human EIF2C3/Ago3 |
| indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human EIF2C3/Ago3 | |
| western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human EIF2C3/Ago3 |
| Application: | Anti-EIF2C3/Ago3 antibody produced in rabbit has been used in immunoprecipitation and western blot assays. |
| Application: | Anti-EIF2C3/Ago3 antibody produced in rabbit has been used: • immunoprecipitation • immunoblotting • immunofluorescence |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)  |
| Biochem/physiol Actions: | Argonaute proteins play a vital role in RNA interference (RNAi). These proteins are also involved in germline development, stem cell fate determination, as well as epigenetic regulation and transposon silencing. Ago3 facilitates the regulation of let-7a-3p expression. Mutation in the gene might lead to the development of Wilms′ tumors. |
| Biochem/physiol Actions: | hAgo2 has been shown to have slicing activity. This region is lost in Wilms′ tumors, which are hypothesized to be caused by defects in embryonic kidney development, which disturb the capacity of metanephrogenic precursor cells to differentiate. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-eukaryotic translation initiation factor 2C 3 (EIF2C3)/protein argonaute-3 (Ago3) is developed in rabbit using as immunogen a synthetic peptide corresponding to human EIF2C3/Ago3 conjugated to KLH. Argonaute proteins (molecular mass 100 kDa) are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of four members, hsAgo1-4. hsAgo2 and Ago3 associate primarily with miRNA. Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1, is associated with the Golgi and endoplasmic reticulum. The gene is located on chromosome 1 in a cluster of closely related family members including Ago 1 and Ago 4. |
| General description: | Argonaute 3, RISC catalytic component (Ago3), also known as eukaryotic translation initiation factor 2C, 3 (EIF2C3), is encoded by the gene mapped to human chromosome 1p34.3. The encoded protein belongs to the argonaute family of proteins and is characterized with a PAZ (Piwi/Argonaute/Zwille) domain and PIWI domains. |
| Immunogen: | synthetic peptide corresponding to amino acids 13-25 of human EIF2C3/Ago3 conjugated to KLH. The corresponding sequence differs by one or two amino acid in rat and mouse, respectively. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |