Monoclonal Anti-FLAG-Peroxidase antibody produced in rat
SIGMA/SAB4200119 - 2-4 mg/mL, clone 6F7, purified immunoglobulin
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human BACE1-FLAG was separated on SDS-PAGE, probed with Monoclonal Anti-FLAG-Peroxidase Clone: 6F7 (Cat. No. SAB4200119) and developed using a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. Negative control: without antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 6F7, monoclonal |
| concentration | 2-4 mg/mL |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein |
| Application: | Learn more product details in our FLAG® applications portal. |
| Application: | Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting. |
| General description: | Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide. |
| General description: | The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. |
| Immunogen: | purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |