Anti-Phospholipase A2 (iPLA2) antibody produced in rabbit
SIGMA/SAB4200129 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-CaI-PLA2; Anti-GVI; Anti-INAD1; Anti-IPLA2-VIA; Anti-PARK14; Anti-PLA2; Anti-PLA2G6; Anti-PNPLA9; Anti-Phospholipase A2, group VI (cytosolic, calcium-independent); Anti-iPLA2
MDL Number: MFCD00803786
Product Type: Chemical
Immunoblotting Cell lysates of HEK-293T overexpressing human iPLA2 (isoform SH-iPLA2) (Lanes 1,2), HEK-293T non-transfected (Lane 3) and RAW264 (Lanes 4,5) were separated on SDS-PAGE, blotted with Anti-Phospholipase A2 (iPLA2) (Cat. No. SAB4200129), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. The observed ~85 kDa corresponds to overexpressed human iPLA2 isoform SH-iPLA2, whereas the ~95 kDa band corresponds to endogenous human and mouse iPLA2 isoform LH-iPLA2. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL + iPLA2 immunizing peptide (human, 541-558) 3. Antibody: 1.5 μg/mL 4. Antibody: 1.5 μg/mL 5. Antibody: 1.5 μg/mL + iPLA2 immunizing peptide (human, 541-558)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~85 kDa (and ~95 kDa human iPLA2 and ~95 kDa mouse and rat iPLA2.) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3.0 μg/mL using using extracts of HEK-293T cells overexpressing human iPLA2, RAW264 cell lysates and rat pancreas extracts (S1 fraction). |
| UniProt accession no. | O60733  |
| Application: | Anti-Phospholipase A2 (iPLA2) antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | Calcium-independent phospholipase A2 (iPLA2) in the absence of Ca2+, catalyzes the hydrolysis of the Sn-2 ester bond in phospholipids to produce free fatty acids and lysophospholipids. iPLA2 might play an essential role in activating the receptor-mediated signaling pathway. Additionally, it also yields precursors for bioactive compounds such as eicosanoid and platelet-activating factor. The encoded protein performs housekeeping role in phospholipid remodeling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Calcium-independent phospholipase A2 (iPLA2) belongs to the group six of phospholipase A2 (PLA2) enzymes. The protein is encoded by the gene mapped to human chromosome 22q13.1. iPLA2 is an intracellular enzyme. It is present both in the cytosol and in membrane fractions. The encoded protein contains 752 amino acids and has a mass of 85kDa. It is characterized with a eight ankyrin repeats and a catalytic domain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |