Anti-ALS2 (N-terminal region) antibody produced in rabbit
SIGMA/SAB4200137 - ~1.5 mg/mL, affinity isolated antibody
Synonym: Anti-ALS2CR6; Anti-ALSIN; Anti-ALSJ; Anti-IAHSP; Anti-PLSJ
Product Type: Chemical
Immunofluorescence Cytoplasmic localization of mouse ALS2 in NIH3T3 cells. Fixed cells were stained with 5 μg/mL Anti-ALS2 (N-terminal region) (Cat. No. SAB4200137) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
Immunoblotting Cell lysates of HEK-293T overexpressing human ALS2 (lanes 1,2) and HEK-293T non transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-ALS2 (N-terminal region) (Cat. No. SAB4200137), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL + ALS2 immunizing peptide (rat, 441-458) 3. Antibody: 1.5 μg/mL
Immunoblotting Rat cerebellum extract (S1 fraction) was separated on SDS-PAGE, blotted with Anti-ALS2 (N-terminal region) (Cat. No. SAB4200137), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL + ALS2 immunizing peptide (rat, 441-458)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~185 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2-4 μg/mL using NIH3T3 cells |
| western blot: 1.5-3.0 μg/mL using HEK-293T cell lysate over expressing human ALS2, and rat cerebellum extract (S1 fraction). |
| Application: | Anti-ALS2 (N-terminal region) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Amyotrophic lateral sclerosis (ALS)2 has been shown to mediate the activation of Rab5 and Rac1/PACK1. In the nervous system, ALS2 is preferentially associated with the cytoplasmic side of endosome membrane, it modulates endosome membrane trafficking and promotes neurite growth in neuronal cultures. Loss-of function mutations in the ALS2 gene leads to the development of recessive motor neuron diseases, including forms of ALS (ALS2), juvenile PLS (PLSJ) and hereditary spastic paraplegia (HSP). |
| Biochem/physiol Actions: | Anti-ALS2 (N-terminal region) specifically recognizes human, rat, and mouse ALS2. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Amyotrophic lateral sclerosis (ALS)2 gene codes for 184 kDa alsin protein. Alsin is characterized with the three putative guanine exchange factor (GEF) domains such as, RCC1-like domain (RLD), the Dbl and pleckstrin homology (DH/PH) domain, and a vacuolar protein sorting 9 (VPS9) domain. In addition, ALS2 also contains eight consecutive membrane occupation and recognition nexus (MORN) motifs. ALS2 mRNA is ubiquitously expressed in the CNS and non-neuronal tissues, with the highest expression in the cerebellum and kidney. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |