Anti-ADAR2 antibody, Mouse monoclonal
SIGMA/SAB4200142 - clone ADAR2-8, purified from hybridoma cell culture
Synonym: Anti-ADARB1; Anti-Adenosine Deaminase, RNA-specific B1; Anti-DRABA2; Anti-DRADA2; Anti-RED1; Anti-RNA Editase 1; Anti-RNA-editing enzyme 1
MDL Number: MFCD02686608
Product Type: Chemical
Immunoblotting Whole extract of HL-60 cells was separated on SDS-PAGE and probed with Mouse Anti-ADAR2 Clone: ADAR2-8 (Cat. No. SAB4200142). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.4 μg/mL antibody 2. 0.4 μg/mL antibody + 40 μg/mL ADAR2 (N-ter peptide) immunizing peptide 3. 0.4 μg/mL antibody + 40 μg/mL ADAR2 (internal peptide) immunizing peptide
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ADAR2-8, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| mol wt | antigen ~81 kDa |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HL-60 total cell extract |
| UniProt accession no. | P78563   |
| Application: | Monoclonal Anti-ADAR2 antibody produced in mouse has been used in immunoblotting. |
| Biochem/physiol Actions: | Adenosine deaminase acting on RNA type 2 (ADAR2) activity is directly involved in both cell cycle by slowing down cell growth rate at the S-G2 phase and in cell migration. Decrease in ADAR2 editing activity is linked to tumor malignancy (astrocytoma grade I-IV) in children. |
| Biochem/physiol Actions: | Adenosine deaminase acting on RNA type 2 (ADAR2) enzyme plays a vital role in editing pre-mRNA of glutamate receptor B subunit. Combined effect of ADAR2 and HTR2C (5-hydroxytryptamine receptor 2C) variants contribute to the suicide attempt (SA) vulnerability in psychiatric patients. ADAR2 is a candidate gene for neurological diseases, such as bipolar affective disorder and epilepsy. Decrease in the activity of ADAR2 enzyme results in |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Adenosine deaminase acting on RNA type 2 (ADAR2), also known as adenosine deaminase, RNA specific B1 (ADARB1), is encoded by the gene mapped to human chromosome 21q22.3. ADARB1 is characterized with two double-stranded RNA-binding domains and a deaminase domain involved in editing action. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |