Anti-ADAR2 antibody, Mouse monoclonal
SIGMA/SAB4200143 - clone ADAR2-15, purified from hybridoma cell culture
Synonym: Anti-ADARB1; Anti-Adenosine Deaminase, RNA-specific B1; Anti-DRABA2; Anti-DRADA2; Anti-RED1; Anti-RNA Editase 1; Anti-RNA-editing enzyme 1
MDL Number: MFCD02686608
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human ADAR2-fusion protein was separated on SDS-PAGE and probed with Mouse Anti-ADAR2 Clone: ADAR2-15 (Cat. No. SAB4200143). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 4 μg/mL antibody 2. 4 μg/mL antibody + 40 μg/mL ADAR2 (N-ter) immunizing peptide 3. 4 μg/mL antibody + 40 μg/mL ADAR2 (internal) immunizing peptide
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | ADAR2-15, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | antigen ~130 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:250-1:500 using HEK-293T cells overexpressing ADAR2 (a working dilution) |
| Application: | Monoclonal Anti-ADAR2 antibody produced in mouse may be used in several immunochemical techniques including immunoblotting. |
| Biochem/physiol Actions: | Adenosine deaminase acting on RNA type 2 (ADAR2) enzyme plays a vital role in editing pre-mRNA of glutamate receptor B subunit. Combined effect of ADAR2 and HTR2C (5-hydroxytryptamine receptor 2C) variants contribute to the suicide attempt (SA) vulnerability in psychiatric patients. ADAR2 is a candidate gene for neurological diseases, such as bipolar affective disorder and epilepsy. Decrease in the activity of ADAR2 enzyme results in |
| Biochem/physiol Actions: | Monoclonal Anti-ADAR2 recognizes human ADAR2 |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Adenosine deaminase acting on RNA type 2 (ADAR2), also known as adenosine deaminase, RNA specific B1 (ADARB1), is encoded by the gene mapped to human chromosome 21q22.3. ADARB1 is characterized with two double-stranded RNA-binding domains and a deaminase domain involved in editing action. |
| General description: | Monoclonal Anti-ADAR2 (mouse IgG2a isotype) is derived from the hybridoma ADAR2-15 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ADAR2, conjugated to KLH. Adenosine deaminase acting on RNA type 2 is expressed mainly in nucleus. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide and horse immunoglobulins |
| Preparation Note: | Store at −20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at −20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |