Anti-Rab18 antibody produced in rabbit
SIGMA/SAB4200173 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-RAB18LI1; Anti-ras-related protein Rab-18
Product Type: Chemical
Immunofluorescence NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab18 (Cat. No. SAB4200173). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of MM3MG cells was separated on SDS-PAGE and probed with Rabbit Anti-RAB18 (Cat. No. SAB4200173). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. 2 μg/mL antibody + RAB18 immunizing peptide
Immunoprecipitation Rabbit Anti-Rab18 (Cat. No. SAB4200173) was used to immunoprecipitate Rab18 from PC3 whole cell extract. Detection antibody: Rabbit Anti-Rab18 (C-terminal) (Cat. No. SAB4200174). Lanes 1. 5 μg/mL antibody 2. 10 μg/mL antibody 3. Negative control: 10 μg/mL non-relevant antibody 4. Positive control: PC3 whole cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~23 kDa (an additional band at ∼43 kDa may appear in some extract preparations) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using lysates of human PC3 cells |
| indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells | |
| western blot: 1-2 μg/mL using whole extracts of mouse MM3MG cells | |
| UniProt accession no. | Q9NP72  |
| Application: | Anti-Rab18 antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Rab guanosine triphosphatases (Rab GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and GTP-bound active states. Activation of a Rab protein is coupled to its association with intracellular membranes. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement and membrane fusion. The conserved regions of Rab proteins are implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. Therefore, antibodies to Rab proteins may serve as useful tool for studying subcellular localization and membrane organization. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rab18 is a member of the Rab family of small guanosine triphosphatases (GTPases). The Rab family belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |