Anti-Rab18 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200174 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-RAB18LI1; Anti-RP11-148B2.1; Anti-ras-related protein Rab-18
Product Type: Chemical
Immunofluorescence NRK cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-Rab18 (C-terminal) (Cat. No. SAB4200174). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Whole extract of MM3MG cells was separated on SDS-PAGE and probed with Rabbit Anti-RAB18 (C-terminal) (Cat. No. SAB4200174). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody 3. 5 μg/mL antibody + RAB18 (C-terminal) immunizing peptide
Immunoprecipitation Rabbit Anti-Rab18 (C-terminal) (Cat. No. SAB4200174) was used to immunoprecipitate Rab18 from PC3 whole cell extract. Detection antibody: Rabbit Anti-Rab18 (Cat. No. SAB4200173). Lanes 1. 2.5 μg/mL antibody 2. 5 μg/mL antibody 3. Negative control: 5 μg/mL non-relevant antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~23 kDa (additional bands of higher MW may appear in some extract preparations) |
| packaging | antibody small pack of 25 μL |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using lysates of human PC3 cells |
| indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells | |
| western blot: 2.5-5.0 μg/mL using whole extracts of mouse MM3MG cells | |
| UniProt accession no. | Q9NP72   |
| Application: | Anti-Rab18 (C-terminal) antibody produced in rabbit may be used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Anti-Rab18 (C-terminal) recognizes human, mouse, and rat Rab18. |
| Biochem/physiol Actions: | Ras-associated binding (Rab)-guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and guanosine triphosphate (GTP)-bound active states. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking. It localizes to lipid droplets and induces the association of the lipid droplets with endoplasmic reticulum (ER) membranes. Anti-Rab18 may thus be used as a marker to follow the dynamics of lipid droplets in living cells. Rab18 has a potential function in eye development. Mutations in the RAB18 are implicated in Warburg micro syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ras-related protein Rab-18 (Rab18) is a member of the ras-associated binding (Rab) family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif, implicated in subcellular targeting. RAB18 gene is mapped to human chromosome 10p12.1. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Discard working dilutions if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |