Anti-Atg101 antibody produced in rabbit
SIGMA/SAB4200175 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-
Product Type: Chemical
Immunoblotting Whole extract of HEK-293T cells overexpressing human ATG101 was separated on SDS-PAGE and probed with Rabbit Anti-ATG101 (Cat. No. SAB4200175). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.25 μg/mL antibody 2. 0.5 μg/mL antibody 3. 0.5 μg/mL antibody + ATG101 immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~30 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101 |
| Application: | Anti-Atg101 antibody has been used in • immunoblotting • immunofluorescence • immunopurification |
| Biochem/physiol Actions: | Autophagy101 (Atg101) is a mammalian Autophagy13 (Atg13) binding protein essential for autophagy. Atg13 forms a stable complex with ULK1 (Serine/threonine-protein kinase ULK1) and FIP200 (FAK family kinase-interacting protein of 200 kDa). Atg101 associates with the ULK1-Atg13- FIP200 complex through direct interaction with Atg13. Mammalian target of rapamycin (mTOR) interacts with the ULK1-Atg13-FIP200 complex in a nutrient dependent manner, suggesting that mTOR regulates autophagy through this complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Autophagy101 (Atg101) is located on the human chromosome at 12q13.13. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |