Anti-Importin 5 (C-terminal) antibody produced in rabbit
SIGMA/SAB4200179 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-IPO5
Product Type: Chemical
Immunofluorescence HEK-293T cells over-expressing Importin 5 were fixed and stained with 5 μg/mL Rabbit Anti-Importin 5 (C-terminal) (Cat. No. SAB4200179) followed by Goat Anti-Rabbit, Cy3 conjugate (×40 magnification).
Immunoblotting Lysate of JURKAT cells was separated on SDS-PAGE and probed with Rabbit Anti-Importin 5 (C-terminal) (Cat. No. SAB4200179). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lanes 1. Antibody 4 μg/mL 2. Antibody 2 μg/mL 3. Antibody 1 μg/mL 4. Antibody 0.5 μg/mL
Immunoprecipitation Rabbit Anti-Importin 5 (Cat. No. SAB4200179) was used to immunoprecipitate human Importin 5 from HEK-293T cell lysate. Detection antibody: Rabbit Anti-Importin 5 (C-terminal) (Cat. No. SAB4200178). Lanes 1. IP antibody:10 μg 2. IP antibody: 5 μg 3. IP antibody: 2.5 μg
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~123 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using lysates of human HEK-293 cells. |
| indirect immunofluorescence: 2.5-5.0 μg/mL using HEK-293 cells overexpressing human Importin 5. | |
| western blot: 2-4 μg/mL using whole extracts of human Jurkat cells. | |
| UniProt accession no. | O00410   |
| Application: | Anti-Importin 5 (C-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Nucleocytoplasmic transport of proteins and RNAs is mediated through nuclear pore complexes (NPCs) and by karyopherins nuclear transport receptors (importins/exportins). Importins mediate either the nuclear import of a very diverse set of protein or RNA cargoes. The function of importins are regulated by the Ran G protein. Ran G protein stimulates the release of cargo from importins. Importins are implicated in anti-aggregation activity of highly basic cargo like histones and ribosomal proteins and in preventing their undesired interactions with other cellular components. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | In human cells, there are more than 20 members of the importins/exportins family. Importin-5 (IPO5) belongs to the importin-β superfamily and is mapped to human chromosome 13q32.2. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |