Anti-Cullin 3 antibody ,Mouse monoclonal
SIGMA/SAB4200180 - clone CUL3-9, purified from hybridoma cell culture
Synonym: Anti-CUL3
MDL Number: MFCD03454920
Product Type: Chemical
Western Blotting JOURNAL CITATION: The CUL3-SPOP-DAXX axis is a novel regulator of VEGFR2 expression in vascular endothelial cells. By: Sakaue, T., Sakakibara, I., et al. in Sci Rep, 2017. PubMed ID: 28216678 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/srep42845), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Whole extracts of Jurkat (A), Mouse Brain (B), and A-20 (C) were separated on SDS-PAGE and probed with Mouse Anti-Cullin 3 Clone: CUL3-9 (Cat. No. SAB4200180). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + Cullin 3 immunizing peptide (human, 751-768)
Immunoprecipitation Monoclonal Anti-Cullin 3 (Cat. No. SAB4200180) was used to immunoprecipitate Cullin 3 from Rat Brain whole extract. Detection antibody: Rabbit Anti-Cullin 3 (C-terminal) (Cat. No. C9745). Lanes 1. 5 μg antibody 2. 10 μg antibody 3. Negative control: 10 μg non-relevant antibody 4. Positive control: Rat Brain whole extract
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CUL3-9, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 80 kDa |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 0.5-1.0 μg/mL using whole extracts of human Jurkat cells or mouse brain. | |
| UniProt accession no. | Q13618   |
| Application: | Monoclonal Anti-Cullin 3 antibody produced in mouse has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | Cullin 3 (CUL3) acts as a scaffold protein and facilitates the organization of a large number of ubiquitin ligase complexes. It plays a vital role in the regulation of electrolyte homeostasis and blood pressure. The encoded protein acts as a key factor in protein ubiquitination and is also implicated in early embryonic development. One important role of CUL3 is to promote invasion and migration of trophoblast cells. Mutation in the gene leads to onset of pre-eclampsia and pseudohypoaldosteronism type II. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cullin 3 (CUL3) is a member of cullin protein family and is encoded by the gene mapped to human chromosome 2q36.2. The encoded protein is a core component of cullin/RING E3 ubiquitin ligase (CRL) and is ubiquitously expressed. The protein is characterized with a cullin homology domain. |
| General description: | Monoclonal Anti-Cullin 3 (mouse IgG1 isotype) is derived from the hybridoma CUL3-9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Cullin 3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |