Anti-Glutathione Reductase antibody, Mouse monoclonal
SIGMA/SAB4200182 - clone GR6, purified from hybridoma cell culture
Synonym: Anti-GR; Anti-GSR; Anti-
MDL Number: MFCD01323367
Product Type: Chemical
Immunoblotting Whole extract of HepG2 cells was separated on SDS-PAGE and probed with Monoclonal Anti-Glutathione Reductase Clone:GR6 (Cat. No. SAB4200182). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 2 μg/mL antibody + Glutathione Reductase recombinant protein 3. 1 μg/mL antibody
Immunoprecipitation Monoclonal Anti-Glutathione Reductase Clone: GR6 (Cat. No. SAB4200182) was used to immunoprecipitate Glutathione Reductase from HepG2 whole cell extract. Detection antibody: Rabbit Anti Glutathione-Reductase (Cat. No. SAB2100980) Lanes 1. 10 μg antibody 2. 5 μg antibody 3. Negative control: 5 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GR6, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~55 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 2-4 μg/mL using whole extracts of human HepG2 cells. | |
| UniProt accession no. | P00390  |
| Application: | Monoclonal Anti-Glutathione Reductase antibody produced in mouse has been used in: • immunohistochemical • immunofluorescence studies • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | Glutathione reductase (GR) is a ubiquitous flavoenzyme involved in protecting cells from stress. GR catalyses the reduction of oxidized glutathione (GSSG) to glutathione (GSH). It is an essential component of the glutathione redox cycle, which maintains adequate levels of reduced cellular GSH. GSH serves as an antioxidant and also as an electron donor for glutathione peroxidases and a substrate for glutathione-S-transferase |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Glutathione Reductase (mouse IgG1 isotype) is derived from the hybridoma GR6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a human Glutathione reductase recombinant protein. Glutathione reductase is a homodimeric enzyme containing one FAD molecule and NADPH binding domain per subunit. This gene is located on human chromosome 8p. |
| Immunogen: | mice immunized with a human Glutathione reductase recombinant protein. The corresponding proteins in rat and mouse share 86% and 85% homology, respectively. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |