Anti-SORBS2 antibody,Mouse monoclonal
SIGMA/SAB4200183 - clone S5C, purified from hybridoma cell culture
Synonym: Anti-ARGBP2; Anti-Arg binding protein 2; Anti-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% v/v Triton™ X-100. Fixed cells were stained with 10 μg/mL Mouse Anti-SORBS2 Clone:S5C (Cat. No. SAB4200183). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cell nuclei were stained with DAPI.
Western Blotting Lysates were separated on SDS-PAGE and probed with 3 μg/mL Mouse Anti-SORBS2, Clone: S5C (Cat. No. SAB4200183). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. L929; 2. 293T; 3. HeLa; 4. HepG2; 5. MCF7; 6. NRK; 7. 3T3; 8. MDCK; 9. CHO
Immunoprecipitation Mouse Anti-SORBS2 antibodies (Cat. No. SAB4200177 and SAB4200183) were used to immunoprecipitate SORBS2 from HeLa whole cell extract. Detection antibody: Rabbit Anti-SORBS2 antibody (Cat. No. SAB4200037). Lanes 1. 1:1,000 antibody Mouse Anti-SORBS2, Clone: S13N (Cat. No. SAB4200177) 2. 1:1,000 antibody Mouse Anti-SORBS2, Clone: S5C (Cat. No. SAB4200183) 3. Negative control: without antibody 4. Positive control: HeLa cell extract
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S5C, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 85 kDa |
| shipped in | dry ice |
| species reactivity | hamster, canine, mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| western blot: 2-4 μg/mL using HeLa or NRK or 3T3 total cell extracts. | |
| UniProt accession no. | O94875   |
| Application: | Monoclonal Anti-SORBS2 antibody is suitable for use in western blot (2-4 μg/mL using HeLa or NRK or 3T3 total cell extracts), immunocytochemistry, and immunoprecipitation. |
| Biochem/physiol Actions: | Sorbin And SH3 Domain Containing 2 (SORBS2) acts as a potential link between Abl kinases and the actin cytoskeleton. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Sorbin And SH3 Domain Containing 2 (SORBS2), also known as ArgBP2, is an Arg/Abl binding protein. It contains an N-terminal sorbin homology (SoHo) domain, that interacts with lipid raft proteins, three C-terminal src homology 3 (SH3) domains, a Ser/Thr-rich domain and several potential Abl phosphorylation sites. SORBS2 associate is a substrate of Arg and v-Abl, which is phosphorylated on tyrosine in v-Abl-transformed cells. SORBS2 is widely expressed in human tissues and is particularly abundant in heart. |
| General description: | SORBS2 or ArgBP2 is an adapter protein that regulates actin-dependent cell adhesion and migration. ArgBP2 facilitates the ubiquitination and degradation of c-Abl. In heart muscle cells, ArgBP2 is localized at Z-disks of sarcomeres and may regulate elasticity of cardiac sarcomeres, whereas in the epithelial cells ArgBP2 may modulate the assembly of signaling complexes in stress fibers . Monoclonal Anti-SORBS2 antibody is specific for SORBS2 (85 kDa) in humans, rats, mice, dogs and hamsters. Staining of the SORBS2 band in immunoblotting is specifically inhibited with the immunizing peptide. |
| Immunogen: | mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C- terminal region of human SORBS2 , conjugated to KLH. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |