Anti-WTX (N-terminal) antibody produced in rabbit
SIGMA/SAB4200184 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-AMER1; Anti-FAM123B; Anti-FLJ39827; Anti-OSCS; Anti-Wilms tumor gene on the X chromosome
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-WTX (N-terminal) antibody produced in Rabbit (Cat. No. SAB4200184) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing WTX (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human WTX (lanes 1,2) and HEK-293T non-transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-WTX (N-terminal) (Cat. No. SAB4200184), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Bands at 190 kDa and 150 kDa respectively correspond to the unspliced and spliced isoforms of WTX. Lanes 1. Antibody: 1 μg/mL 2. Antibody: 1 μg/mL + immunizing WTX peptide 3. Antibody: 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~190 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cell lysates over expressing human WTX. |
| Application: | Anti-WTX (N-terminal) antibody produced in rabbit has been used in chromatin immunoprecipitation and immunoblotting. |
| Biochem/physiol Actions: | Anti-WTX (N-terminal) specifically recognizes human WTX. |
| Biochem/physiol Actions: | APC membrane recruitment protein 1 (AMER1) or WTX is localized in the subnuclear compartment and participates in RNA processing, transcription and cell differentiation. In cultured cells, WTX negatively regulates the WNT/β-catenin signaling by promoting β-catenin ubiquitination and thus targets its degradation by the proteasome. WTX has been shown to form a complex with β-catenin, Axis inhibition protein 1 (AXIN1), β-transducin repeat containing protein 2 (β-TrCP2) and adenomatous polyposis coli (APC). WTX mutations are majorly somatic and the gene deletions and truncations is implicated in 30% of Wilms tumors. The mutations were confined to the active X and single X chromosomes in females and males respectively.. Loss of WTX function in Wilms tumor results in stabilization of β-catenin and its translocation to the nucleus. WTX co-localizes with non-POU domain-containing octamer-binding protein (p54nrb/NONO) and binds Wilms′ tumor protein 1 (WT1) to modulate its activity, suggesting that WTX may play a role in the transcriptional regulation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | APC membrane recruitment protein 1 (AMER1) or Wilms Tumor on the X (WTX) gene, also known as FAM123B, comprises adenomatous polyposis coli (APC) interacting domains, nuclear localization signal (NLS), phospholipid binding region, six conserved block sequences and β-catenin binding region. The N-terminal region harbors the internal splicing sites (donor and acceptor). It encodes three isoforms AMER1, AMER2 and AMER3 APC, by alternative splicing at the N-terminus. WTX gene is localized on the X chromosome. The phosphatidylinositol (4,5)-bisphosphate binding site of the WTX aids its localization to the plasma membrane. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |