Anti-Sumo-1 antibody, Rat monoclonal
SIGMA/SAB4200189 - clone 4D12, purified from hybridoma cell culture
Synonym: Anti-DAP1; Anti-GMP1; Anti-OFC10 CLEFT LIP WITH OR WITHOUT CLEFT PALATE, NONSYNDROMIC, 10; Anti-SMT3 suppressor of mif two 3 homolog 1 SENTRIN PIC1 OROFACIAL CLEFT 10, INCLUDED; OFC10; Anti-SMT3H3 SMT3C; Anti-UBL1, SMT3, YEAST, HOMOLOG 3; Anti-small ubiquitin-like modifier 1; Anti-ubiquitin-like 1; Monoclonal Anti-Sumo-1; SUMO-1; SUMO1
MDL Number: MFCD02096449
Product Type: Chemical
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Rat Anti-Sumo1 Clone: 4D12 (Cat. No. SAB4200189). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. Negative control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | 4D12, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | ~12 kDa |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: 2-4 μg/mL using HeLa cell extracts | |
| UniProt accession no. | P63165   |
| Application: | Monoclonal Anti-Sumo-1 has been used in immunocytochemistry. |
| Application: | Monoclonal Anti-Sumo-1 has been used in western blotting. |
| Biochem/physiol Actions: | Sumo-1 interacts with dsDNA and competes with other protein-DNA interactions. |
| Biochem/physiol Actions: | Suppressing SUMO1 (small ubiquitin-related modifier) inhibits multiplication and induces apoptosis of gastric cancer SGC-7901 cells. It is essential for HCV (hepatitis C virus) replication. Deletion of SUMO1 blocks the aggregation of lipid droplets. It is also implicated in mitochondrial fission. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Sumo-1 (rat IgG2a isotype) is derived from the hybridoma 4D12 produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from rat immunized with a fusion protein of human SUMO1. |
| General description: | SUMO1 (small ubiquitin-related modifier), also called as Pic1 (inner centromere protein-related protein), Ubl1 (ubiquitin-like protein), hSmt3 (human homolog 3), or sentrin, is mainly expressed in the nucleus. The gene encoding SUMO1 is mapped to human chromosome 2q33. SUMO1 belongs to ubiquitin-like protein family and it consists of 101 amino acids. |
| Immunogen: | fusion protein of human SUMO1 |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |