Anti-Sumo-2/3 antibody, Rat monoclonal
SIGMA/SAB4200190 - clone 3H12, purified from hybridoma cell culture
Synonym: Anti-HSMT3; Anti-MGC117191; Anti-SMT3 homolog 2; Anti-SMT3 suppressor of mif two 3 homolog 2; Anti-SMT3B; Anti-SMT3H2; Anti-sentrin 2; Anti-small ubiquitin-like modifier 2; Anti-small ubiquitin-related modifier 2; Anti-ubiquitin-like protein SMT3B; Monoclonal Anti-Sumo-2/3; SUMO-2/3; SUMO2/3
MDL Number: MFCD03455941
Product Type: Chemical
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Rat Anti-Sumo2/3 Clone: 3H12 (Cat. No. SAB4200190). The antibody was developed using Rabbit Anti-Rat IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. Negative control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone | 3H12, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | ~11 kDa |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: 2-4 μg/mL using HeLa cell nuclear extract | |
| UniProt accession no. | P55854   |
| Application: | Monoclonal Anti-Sumo-2/3 has been used in immunoblotting and immunocytochemistry. |
| Biochem/physiol Actions: | Small ubiquitin-like modifier (SUMO) was identified as a post-translational protein modifier. |
| Biochem/physiol Actions: | Small ubiquitin-like modifier 3 (SUMO3) plays a vital role in enhancing cellular responses to environmental stress. It acts as a key element of the leukemogenic network in transient myeloproliferative disorder (TMD) of the newborn/acute megakaryoblastic leukaemia (AMKL). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Sumo-2/3 (rat IgG2a isotype) is derived from the hybridoma 3H12 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a human SUMO3 fusion protein. |
| General description: | Small ubiquitin-like modifier 3 (SUMO3) is encoded by the gene mapped to human chromosome 21q22.3. The protein belongs to the ubiquitin-like protein family and is expressed mainly in cytoplasm. |
| Immunogen: | human SUMO3 fusion protein |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |