Anti-Septin 9 antibody, Rat monoclonal
SIGMA/SAB4200191 - clone Clone 1A6C2, purified from hybridoma cell culture
Synonym: Anti-MLL; Anti-MSF; Anti-MSF1; Anti-NAPB; Anti-PEANUT-LIKE 4; Anti-PNUTL4; Anti-SEPT9; Anti-SINT1; Anti-SeptD1; Monoclonal Anti-Septin 9 antibody produced in rat
Product Type: Chemical
Immunoblotting Lysates were separated on SDS-PAGE and probed with 5 μg/mL Rat Anti-Septin9 Clone: 1A6C2 (Cat. No. SAB4200191). The antibody was developed using Rabbit Anti-RAT IgG-Peroxidase (Cat. No. A9542) and a chemiluminescent substrate. Lanes 1. L929 2. 3T3 3. NRK 4. HeLa 5. COS-7
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | Clone 1A6C2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | ~72 kDa |
| shipped in | dry ice |
| species reactivity | human, rat, monkey, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| western blot: 2.5-5.0 μg/mL using L929, HeLa, 3T3 and COS-7 cell extracts |
| Application: | Monoclonal Anti-Septin 9 has been used in: • immunoblotting • immunocytochemistry • immunohistochemistry • immunofluorescence |
| Biochem/physiol Actions: | Septin 9 (SEPT9) has been implicated in tumorigenesis. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. In addition, SEPT9 is shown to be a DNA methylation-based biomarker, effectively discriminated colorectal cancer from normal specimens. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Septin 9 (rat IgG2a isotype) is derived from the hybridoma 1A6C2 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a synthetic peptide of corresponding to the N-terminus of rat Septin 9, conjugated to KLH. Septin 9 (SEPT9) is a member of the septin family (also designated MSF/eseptin/Sint1). This gene is mapped to human chromosome 17q25. |
| Immunogen: | synthetic peptide of corresponding to the N-terminus of rat Septin 9 conjugated to KLH |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |