Anti-Cyclophilin B antibody produced in rabbit
SIGMA/SAB4200201 - ~1.0 mg/mL, affinity isolated antibody
Synonym: Anti-CYP-S1; Anti-CYPB; Anti-OI9; Anti-PPIB; Anti-PPIase B; Anti-S-cyclophilin; Anti-SCYLP; Anti-
MDL Number: MFCD02265740
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 10 μg/mL Anti-Cyclophilin B antibody produced in Rabbit (Cat. No. SAB4200201) (A). The antibody developed using Goat Anti-Rabbit IgG, Atto 448 Conjugate antibody, and 5 μg/mL Anti-Cyclophilin B antibody, Mouse monoclonal, Clone: CYPB-3 (Cat. No. SAB4200360) (B). The antibody was developed using Rabbit Anti-Mouse IgG, Cy3 Conjugate antibody. Results Two Anti-Cyclophilin B antibodies, SAB4200201 (A) and SAB4200360 (B), target different regions of Cyclophilin B show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence 3T3 cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 10 μg/mL Rabbit Anti-Cyclophilin B (Cat. No. SAB4200201). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-Cyclophilin B (Cat. No. SAB4200201). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. HepG2; 2. A431; 3. HeLa; 4. NRK; 5. Hepa 1-6; 6. Mouse Liver; 7. COS7; 8. Rat2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | ~20 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, rat, monkey, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells |
| western blot: 0.5-1.0 μg/mL using whole extracts of human HepG2 and rat NRK cells | |
| UniProt accession no. | P23284  |
| Application: | Anti-Cyclophilin B antibody produced in rabbit has been used: • immunoblotting • immunofluorescence • in immunocapture-polymerase chain reaction (PCR) to study the interactions between CypB and Tomato yellow leaf curl virus (TYLCV) coat protein (CP). |
| Biochem/physiol Actions: | Cyclophilins are peptidyl-prolyl cistrans isomerases (PPIases) that assist protein folding by catalysing the isomerization of peptidyl-proline bonds. Cyclophilin B (CypB) also plays an important role in protecting cells against ER stress via its PPIase activity. Mutations in the PPIB gene give rise to recessive forms of osteogenesis imperfecta and knockdown of this gene decreases cell growth, proliferation and migration. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Cyclophilin B is produced in rabbit using as the immunogen a synthetic peptide corresponding to a fragment of human Cyclophilin B conjugated to KLH. Cyclophilin B (CypB) is a 21 kDa protein encoded by the PPIB gene, that belongs to the cyclophilin family, a highly conserved class of proteins originally identified as cellular binding proteins for the immunosuppressive drug cyclosporin A. CypB is mainly located within the endoplasmic reticulum (ER) of all cell types. CypB is involved in inflammation, viral infection and cancer. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |