Anti- INI1/SNF5 antibody, Mouse monoclonal
SIGMA/SAB4200202 - clone 2C2, purified from hybridoma cell culture
Synonym: Anti-BAF47; Anti-RDT; Anti-RTPS1; Anti-SMARCB1; Anti-SNF5L1; Anti-SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1; Anti-Sfh1p; Anti-Snr1; Anti-hSNFS
MDL Number: MFCD04118401
Product Type: Chemical
Immunoblotting HeLa cell extract was separated on SDS-PAGE and probed with Mouse Anti-INI1/SNF5 Clone: 2C2 (Cat. No. SAB4200202). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2.5 μg/mL antibody 2. 1.0 μg/mL antibody 3. 0.5 μg/mL antibody 4. Negative control
Immunoblotting Lysates were separated on SDS-PAGE and probed with 1.0 μg/mL Mouse Anti-INI1/SNF5 Clone: 2C2 (Cat. No. SAB4200202). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HeLa; 2. A549; 3. 3T3; 4. Neuro 2a; 5. PC12; 6. COS-7; 7. MDCK; 8. MDBK
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2C2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~44 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, bovine, canine, mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| western blot: 1.0-2.0 μg/mL using HeLa cell extracts | |
| UniProt accession no. | Q12824  |
| Application: | Monoclonal Anti-INI1/SNF5 antibody produced in mouse has been used in immunoblotting and immunocytochemistry. |
| Biochem/physiol Actions: | INI1/sucrose non-fermentable 5 (SNF5) plays a vital role in transcriptional activation of inducible genes through chromatin remodelling. Loss of function mutations of the gene is associated with the pathogenesis of aggressive pediatric cancer and epithelioid schwannoma. The encoded protein triggers DNA-joining activity of the HIV-1 integrase in vitro. Additionally, it might also help in targeting the viral DNA to active genes. SNF5 interacts with E1 protein of human papillomavirus (HPV) and thus, enhances the efficiency of HPV DNA replication. |
| Biochem/physiol Actions: | Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) is recruited to HIV-1 preintegration complexes before nuclear migration and represses the basal human immunodeficiency virus type 1 promoter activity. This gene is a tumor suppressor and mutations in it have been associated with various tumors. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | INI1/SNF5, also known as SWItch/sucrose non-fermentable (SWI/SNF) related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1, is encoded by the gene mapped to human chromosome 22q11.2. This gene codes for an essential component of chromatin-remodelling SWI/SNF multiprotein complexes. |
| General description: | Monoclonal Anti-INI1/SNF5 (mouse IgG2a isotype) is derived from the hybridoma 2C2 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from mouse immunized with a human INI1 fusion protein. Integrase interactor 1 (INI1)/sucrose non-fermentable 5 (SNF5) binds to human immunodeficiency virus type 1 (HIV-1) integrase. |
| Immunogen: | human INI1 fusion protein. |
| Physical form: | Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |