Anti-VPS4A antibody, Mouse monoclonal
SIGMA/SAB4200215 - clone VPS4-110, purified from hybridoma cell culture
Synonym: Anti-SKD1, SKD1A, SKD2, VPS4-1; Anti-Vacuolar protein sorting factor 4A; Anti-hVPS4
Product Type: Chemical
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-VPS4A Clone: VPS4-110 (Cat. No. SAB4200215). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T; 2. NRK; 3. NIH-3T3; 4. BGME; 5. PC12
Immunoprecipitation Monoclonal Anti-VPS4A Clone: VPS4-110 (Cat. No. SAB4200215) was used to immunoprecipitate VPS4A from HEK-293T whole cell extract. Detection antibody: Rabbit Anti-VPS4 (Cat. No. SAB4200025) Lanes 1. 5 μg antibody 2. 2.5 μg antibody 3. Negative control: 5 μg non-relevant antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VPS4-110, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| shipped in | dry ice |
| species reactivity | mouse, canine, bovine, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| western blot: 1-2 μg/mL using whole extracts of mouse 3T3 or human HEK-293 cells. | |
| UniProt accession no. | Q9UN37   |
| Application: | Monoclonal Anti-VPS4A antibody has been used in • immunoblotting • immunoprecipitation • immunolabelling |
| Biochem/physiol Actions: | Vacuolar-protein-sorting factor 4A (VPS4A) plays a vital role in lysosomal/endosomal membrane trafficking. It is also involved in the regulation of exosome biogenesis. Downregulated expression of the gene leads to the development of hepatocellular carcinoma (HCC). Thus, VPS4 is considered to be an effective tumor suppressor for HCC. Mutation of the gene leads to defective multivesicular body formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-VPS4A (mouse IgG1 isotype) is derived from the hybridoma VPS4-110 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human VPS4A, conjugated to KLH. |
| General description: | Vacuolar-protein-sorting factor 4A (VPS4A) is an AAAtype ATPase, encoded by the gene mapped to human chromosome 16q22.1. The encoded protein is a member of vacuolar sorting protein family. The protein is characterized by a canonical AAA ATPase cassette followed by an N-terminal MIT (microtubule interacting and trafficking) domain. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |