Monoclonal Anti-Caveolin-1 antibody produced in mouse
SIGMA/SAB4200216 - clone CAV1, tissue culture supernatant
Synonym: Anti-BSCL3; Anti-CAV1; Anti-CGL3; Anti-MSTP085; Anti-VIP21; Anti-caveolae protein, 22kDa
MDL Number: MFCD01633097
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded human lung sections with 1:100 Monoclonal Anti-Caveolin 1 Clone: CAV1 (Cat. No. SAB4200216) using biotin/ExtrAvidin®-Peroxidase.
Immunofluorescence 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:1,000 Monoclonal Anti-Caveolin 1 Clone: CAV1 (Cat. No. SAB4200216). The antibody was developed using Goat Anti-Mouse IgG, Atto 488 conjugate (Cat. No. 62197). Cells were counterstained with DAPI (blue) to stain nuclei (×100 magnification).
Enhanced Validation-By Independent Antibodies Immunofluorescence. 3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Anti-Caveolin-1 antibody produced in Rabbit (Cat. No. C3237) (B). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1:1,000 Monoclonal Anti-Caveolin-1 antibody produced in Mouse, Clone: CAV1 (Cat. No. SAB4200216) (A). The antibody was developed using Goat Anti-Mouse IgG, Atto 448 conjugate antibody. Results Two Anti-Caveolin-1 antibodies, SAB4200216 (A) and C3237 (B), target different regions of Caveolin-1 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting Whole extract of Rat1 cells was separated on SDS-PAGE and probed with Monoclonal Anti-Caveolin 1 Clone: CAV1 (Cat. No. SAB4200216). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1:500 antibody 2. 1:500 antibody + immunizing peptide (human, 2-20) 3. 1:1,000 antibody
| antibody form | tissue culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CAV1, monoclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| isotype | IgM |
| mol wt | antigen 22 kDa |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| indirect immunofluorescence: 1:1000-1:2000 using human HUVEC or mouse 3T3 cells. | |
| western blot: suitable | |
| UniProt accession no. | Q03135  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Caveolin-1 antibody has been used in western blot analysis. |
| Biochem/physiol Actions: | Caveolin-1 may play a major role in the growth and evolution of cancer. It participates in glucose metabolism. It may also participate in angiogenesis. Cav-1 helps to control the expression of glucose transporters. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caveolin-1 (CAV1) is a multifunctional scaffolding protein, that has three exons. It is located on human chromosome 7q31. |
| Immunogen: | synthetic peptide corresponding to a sequence at the N-terminal of human caveolin-1, conjugated to KLH. |
| Physical form: | Culture supernatant solution containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |